COVID-19 Testing


Summary of MUSC SARS-CoV-2 Assays

Characteristic BD Veritor plus Abbott IDnow BioFire Respiratory Panel RP2.1 Cepheid Express Abbott m2000/Alinity m
Technique

Immuno-chromatographic assay

Isothermal NEAR amplification
Real-time nested multiplex PCR
Real-time PCR
Real-time PCR
Detects  
SARS-CoV-2 Antigen
SARS-CoV-2 RNA
Adenovirus
Coronaviruses
SARS-CoV-2
Metapneuovirus
Rhinovirus/Enterovirus
Influenza A
Influenza B
Parainfluenza
RSV
B. parapertussis
B. pertusis
Chlamydia pneumonia
Mycoplasma pneumonia

SARS-CoV-2 RNA

SARS-CoV-2 RNA
Point(s) of service
POCT  
POCT  
in lab
POCT and in lab
in lab
Analysis time
15 min
5 to 15 min
1 hr
25-36 min
8 hr (m2000), 2-6 hr (Alinity)
Turnaround Time

not stated, performed at point of care

not stated, performed at point of care

2 hr

2 hr

24 hr

Specimen 

Dry nasal swab

Dry nasal swab or NP swab

NP swab in 3 ml UTM

NP swabs in 3ml UTM or saline

NP swabs in 3ml UTM or saline;

Mid-Turbinate (MT) swab in 3 ml UTM or saline

EPIC lab number

POC 310

POC 309

LAB 2081

POC 311

LAB 9193

LAB 8572

Best use case scenarios

Symptomatic individuals

Symptomatic individuals (asymptomatic?)

Symptomatic individuals

Symptomatic and asymptomatic individuals

Symptomatic and asymptomatic individuals

Limit of detection (copies/ml)

3,783,780

(estimated)

500

330 genomic copies/mL

250

100

Sensitivity  84% 94.4% 97.4% 97.8% 100%
Specificity  100% 99.3% 99.4% 95.6% 96.5%

 

There are multiple COVID-19 tests available to detect the SARS-CoV-2 virus. There are rapid tests, which look for antigens that will indicate if a patient is actively infected with SARS-CoV-2. These antigen tests are rapid, but often less sensitive than a molecular test. Rapid tests also increase the possibility of a false positive and are most sensitive within the first days of symptom onset. Molecular tests, also referred to as nucleic acid amplification tests, include extraction of RNA from the patient and them amplification using polymerase chain reaction (PCR). The possibility of getting a false positive is lowest with a molecular test.

Retired Molecular Diagnostic Assays:

  • BioFire COVID-19 Assay, no crossing threshold value available
  • ThermoFisher TaqPath Combo SARS-CoV-2 assay
  • Hologic Aptima SARS-CoV-2 assay (transcription-mediated amplification), no crossing threshold value available
  • Cepheid Express 4-Plex assay (Flu A/B, RSV, SARS-CoV-2), retired on 7/21/2021

 

COVID-19 testing began at MUSC on March 23rd, 2020.

 

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